Fitc staining protocol
WebPrint this indirect flow cytometry protocol. Download our membrane staining summary. Indirect labeling requires two incubation steps, firstly with a primary antibody, then with a … WebGeneral procedure for flow cytometry using a conjugated primary antibody. Print this protocol. Harvest, wash the cells, and adjust cell suspension to a concentration of 1-5 x 10 6 cells/mL in ice-cold PBS, 10% FCS, 1% …
Fitc staining protocol
Did you know?
WebAcrosome membrane integrity was evaluated following the protocol of ref. 54 , in which sperm were stained with PNA-FITC (final concentration: 1.2 µM) for 5 min at 38°C in the … WebOct 8, 2013 · Here is the protocol for preparation and imaging of bacteria for fluorescence microscopy (Growth Curve protocol--see Step 9 under Section II). I also attached a protocol for FITC labeling of bacteria, which you will need once you start the phagocytosis assays. I’m not emailing the phagocytosis protocol since we will probably need to …
Web5e5 of PBMCs were stained with Alexa Fluor 647-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HA2H6) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested). Protocol WebWe recommend typical dilutions as follows: Imaging applications—1:500 to 1:1000; Flow cytometry— 1:2000 or higher. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. for the fluorophore- and biotin-labeled antibodies, including the phycoerythrin-, allophycocyanin-, and tandem …
WebApr 13, 2024 · Extracellular vesicles have shown good potential in disease treatments including ischemic injury such as myocardial infarction. However, the efficient production of highly active extracellular ... WebThe following protocol combines BrdU (FITC) staining of S phase cells in combination with propidium iodide for cell cycle analysis. This protocol is used courtesy of Prabhat …
WebFITC ANNEXIN V STAINING & BLOCKING PROTOCOL . FITC Annexin V is used to quantitatively determine the percentage of cells within a population that are actively undergoing apoptosis. It relies on the …
WebFITC anti-human CD55 Antibody - CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein also known as decay-accelerating factor (DAF). ... For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. ... Cell Surface ... optumotcuhc.conveyhs.comWebTransfer 100 µL of cell suspension in a 5 mL test tube. 3. Add 5 µL of FITC Annexin V. 4. Add 5 µL of 7-AAD Viability Staining Solution. 5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark. 6. Add … optuminsight life sciencesWebFixable Dyes - Dead cells allow fixable viability dyes to cross their membranes where they stain intracellular amines that are more abundant in the cytoplasm than the extracellular amines on the surface of live cells. Cells can be formaldehyde fixed post staining. Cells stained with these products can also be run unfixed. optuminsight management consulting companyWebMethod. 1. Cell incubation with Annexin V-FITC. 1.1 Induce apoptosis via the desired method. 1.2 Collect 1–5 x 10 5 cells by centrifugation. 1.3 Resuspend cells in 500 µL of … optuminsight provider phone numberWebApplication Notes. Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections 5 and formalin-fixed paraffin-embedded tissue sections. optuminsight numberWebThe nuclei were counterstained with blue fluorescent Hoechst 33342 nucleic acid stain. The image was acquired using bandpass filter sets appropriate for the Texas Red dye, fluorescein, and DAPI. The image was acquired … portside low sectionalWebThe protocol suggests 40-80 µg per mg of antibody; for a first-time titration of FITC, try a range of 10 to 400 µg FITC per mg of antibody (for instance, 10, 40, 80, 160, 320 µg per … portside review poetry